Effect of nitrogen addition on the carbon metabolism of soil microorganisms in a Calamagrostis angustifolia wetland of the Sanjiang Plain, northeastern China

Abstract Purpose Soil microorganisms are important mediators of land ecosystem functions and stability. However, carbon sources in different amounts nitrogen addition known to affect the function soil microbial communities. Thus, this study sought evaluate effects on utilization capacity Sanjiang Plain wetland, northeastern China. Methods Three treatments (CK, 0 kg N ha −1 a ; N40, 40 N80 ) were evaluated Honghe National Nature Reserve Plain. The metabolism C. angustifolia wetland was investigated after five consecutive year’s treatment using Bio-Eco technique. Results Different conditions resulted significant differences pH, ammonium (NH 4 + ), dissolved organic (DOC), alpha diversity. average well-color development (AWCD) Plate assay increased gradually with incubation time, levels significantly affected these AWCD values ( P < 0.05), N40 exhibiting highest value. Furthermore, had lower Shannon Pielou diversity indices 0.05). promoted carbohydrate, amino acid, ester rates by microorganisms, whereas inhibited alcohol, amine, acids utilization. Redundancy analysis (RDA) showed that three remarkable community metabolism, cumulative variance contribution 72.86%. In addition, RDA revealed positively correlated TN, SMC, DON, TOC but negatively DOC, NH , NO 3 − . Conclusion Long-term leads changes structure alters ability utilize Calamagrostis wetland.